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  Table of Contents  
ORIGINAL ARTICLE
Year : 2020  |  Volume : 41  |  Issue : 1  |  Page : 93-96
 

Profile of intestinal parasitic infections in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Northeast India


Department of Microbiology, Military Hospital, Shillong, Meghalaya, India

Date of Submission12-Dec-2017
Date of Acceptance24-Sep-2019
Date of Web Publication18-Jun-2020

Correspondence Address:
Dr, Mohammed Ashraf Ali S Namaji
Qtr No 112/4, Pine Walk Area, Near Lady Keane College, Shillong Cantt, Shillong - 793 001, Meghalaya
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijstd.IJSTD_115_17

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   Abstract 

Introduction: Diarrhea is one of the major complications occurring in over 90% of human immunodeficiency virus (HIV)-infected individuals in developing countries. Coccidian group of parasitic infections remain the standout opportunistic pathogens in many parts of the world. Aim: The objective was to understand the profile of diarrheagenic parasites in HIV/AIDS patients along with analysis of the changing trends in the profile of parasitic diarrhea with special context to coccidian parasitic infections. Methodology: A cross-sectional study was performed at “ID CENTRE FOR NORTHEAST,” Shillong, from January 2014 to October 2017. Stool samples collected were observed microscopically for parasites both on direct and concentrated stool samples under ×10 and ×40 magnification. Modified acid-fast staining was used for the detection of coccidian parasites. All statistical analyses were performed using IBM SPSS software, Version 24.0. Results: The prevalence of intestinal parasitic infections was 40.99%, coccidian parasitic infection accounted for 85.13% of total intestinal parasitic infections. Cryptosporidium parvum was the most common cause of diarrhea (70.64%), followed by Cystoisospora belli (23.81%) and Cyclospora spp. (5.55%). Trend analysis of coccidian etiology during the study revealed a significant rise in the positivity of Cryptosporidium spp. and a decrease in the Cystoisosporiasis belli infection. The common noncoccidian parasites identified include hookworm (8.1%) followed by Ascaris lumbricoides (4.7%). Conclusion: The magnitude of parasitic infections is considerably high among the HIV/AIDS patients in Northeast India, and it is essential for screening and periodic monitoring of all the HIV patients for coccidian parasites by stool microscopy.


Keywords: HIV and intestinal parasites, HIV in Northeast India, trends in coccidian parasitic infections


How to cite this article:
Namaji MS, Pathan SH, Balki AM. Profile of intestinal parasitic infections in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Northeast India. Indian J Sex Transm Dis 2020;41:93-6

How to cite this URL:
Namaji MS, Pathan SH, Balki AM. Profile of intestinal parasitic infections in human immunodeficiency virus/acquired immunodeficiency syndrome patients in Northeast India. Indian J Sex Transm Dis [serial online] 2020 [cited 2020 Aug 14];41:93-6. Available from: http://www.ijstd.org/text.asp?2020/41/1/93/287000



   Introduction Top


With the emergence of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) worldwide, a group of human opportunistic pathogens has come into prominence.[1] Intestinal parasites, especially the opportunistic pathogens, are the common cause of morbidity and mortality, with chronic diarrhea being the most common clinical presentation.[2],[3] Reports indicate that diarrhea occurs in 30%–60% of AIDS patients in developed countries and in about 90% of AIDS patients in developing countries.[4] Common parasites associated with diarrhea in HIV-infected persons are, either well-established enteric pathogens, e.g., Entamoeba histolytica, Giardia lamblia, and Strongyloides stercoralis or an opportunistic pathogen, e.g., Cryptosporidium, Isospora, Cyclospora, and Microsporidia.[5],[6]

In India, it is a well-known fact that parasitic diarrheal diseases in HIV/AIDS patients is on the rise in the past three decades owing to numerous studies done on it. Although the Northeast India is the highest contributor of HIV/AIDS cases, not many studies are done to explore the prevalence of opportunistic intestinal infections in such patients.[7]

In view of this, the present study is done to understand the profile of diarrheagenic parasites in these patients along with analysis of the trends in the prevalence of these organisms in the past 4 years.


   Methodology Top


A descriptive cross-sectional study was done from January 2014 to October 2017, at the ID Center, Shillong, which provides clinical care to around 2000 registered HIV patients of entire Northeast India. A total of 361 patients were enrolled in the study as per the inclusion criteria. HIV patients who had not received any antibiotic and antiprotozoal drugs in the past 3 weeks and having acute, chronic, or intermittent diarrhea were included in the present study.

Sample collection

Stool samples were collected in clean wide-mouthed, leak-proof plastic containers from each patient and observed macroscopically for consistency, presence of mucus, pus, blood, adult worms and their segments, and helminthic larvae. Microscopy was done on both direct and concentrated samples under ×10 and ×40 magnification, and formol-ether sedimentation technique was used to concentrate the parasitic ova and cysts. To detect the intestinal coccidian parasites, smears prepared from stool samples were stained with modified acid-fast method with 1% acid-alcohol as decolorizer.

Sample collection was done only once. Results were tabulated in Microsoft Excel sheet and analyzed using IBM Corp. Released 2016. IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp.


   Results Top


Of the 361 patients enrolled, 218 were male and 143 were female. Age group of the patients was ranging from 11 to 60 years. Maximum numbers of patients were in age group of 21–30 years followed by 31–40 years in both sexes.

Parasitic cause was attributed in 40.99% of cases (n = 148). Coccidian parasites accounted for 85.13% of these parasitic infections and remaining 14.87% were due to noncoccidian parasites. Of the coccidian parasites, Cryptosporidium parvum was found to be the most common cause [Table 1] and [Figure 1].
Table 1: Pathogens associated with diarrhea in the study group

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Figure 1: Parasites causing diarrhea in the study group (in percentage)

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A gradual year-wise increase in the incidence of coccidian parasitic infection was observed, with C. parvum being the most common cause (70.64%) and Cyclospora spp. being the least common (5.55%) [Figure 2]. Analysis of trends of prevalence of various coccidian parasites shows a rise in the prevalence of C. parvum infection and sudden decrease in Cystoisospora infection [Figure 3].
Figure 2: Prevalence of the coccidian parasitic infections in the study group

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Figure 3: Analysis of trends of three common coccidian parasitic infections since 2014

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   Discussion Top


Continued progression of AIDS pandemic and its association with intestinal parasitic infections is now a serious concern in India, especially Northeast India which is the worst affected and least explored.[8],[9]

In these patients, enteric infections occur with increased frequency, and some of these are more likely to be persistent, severe, recurrent, and associated with extraintestinal manifestations.[6]

The decrease in immunity by attack on the immune system, especially the cluster of differentiation (CD4+ T-cells) component, macrophages, and defect in the production of immunoglobulin A increases susceptibility to these parasites.[2],[10]

Our study shows the prevalence of parasitic infections to be 40.99%. It is in concordance with the prevalence rates in different parts of India within the past two decades[Table 2].[11],[12],[13],[14] In our study, the most common parasite was C. parvum and was found in 60.13% of cases, Anand et al. from Manipur in 1996 reported the prevalence of Cryptosporidium as 46.6%.[15] There is an increase in 14.47% of cases in the past two decades in Northeast India. Cryptosporidium was found to be the etiological agent of diarrhea in 10%–20% of patients with AIDS worldwide.[6] Biological factors that impact the epidemiology of Cryptosporidium include their low infective dose (10–100 oocysts), ubiquitous nature, small (4–5 μm) size, environmentally and chemically resistant sporulated/infectious oocysts when passed. Due to the lack of effective therapy for cryptosporidiosis and resistance to available drugs, the prognosis is poor in HIV/AIDS patients.[1]
Table 2: Prevalence of intestinal parasitic infections in HIV/AIDS patients in various parts of the country

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In our study, the prevalence of Cystoisospora belli infection was 20.29% which is very high compared to reports across various parts of India and the developing world, a study done by Swathirajan et al.[16] at Chennai, South India, shows the prevalence of C. belli to be 60.9%.

The difference in the prevalence of intestinal parasitic infestations can be attributed to the difference in the geographical distribution of parasites, their personal hygiene, and sanitary habits.[6]


   Conclusion Top


The magnitude of parasitic infections is considerably high among the HIV/AIDS patients in Northeast India, and it is essential for screening and periodic monitoring of all the HIV-seropositive patients for coccidian parasites by stool microscopy. From patients' perspective, avoiding these infections relies on adhering to proper treatment regimens and maintaining good personal hygiene such as drinking boiled water, which may help in avoiding the risk of diarrheal disorders.[16]

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Arora DR, Arora B. AIDS-associated parasitic Diarrhoea. Indian J Med Microbiol 2009;27:185-90.  Back to cited text no. 1
[PUBMED]  [Full text]  
2.
Obateru OA, Bojuwoye BJ, Olokoba AB, Fadeyi A, Fowotade A, Olokoba LB. Prevalence of intestinal parasites in newly diagnosed HIV/AIDS patients in Ilorin, Nigeria. Alex J Med 2017;53:111-6.  Back to cited text no. 2
    
3.
Ibrahim A, Ikeh E, Malu A, Okeke E, Damen J. Intestinal parasitosis in Human Immunodeficiency Virus (HIV) infected adults with chronic Diarrhoea at Jos University Teaching Hospital, Nigeria. Internet J Parasitic Dis 2007;2(1):1559-4629.  Back to cited text no. 3
    
4.
Kulkarni SV, Kairon R, Sane SS, Padmawar PS, Kale VA, Thakar MR, et al. Opportunistic parasitic infections in HIV/AIDS patients presenting with Diarrhoea by the level of immunesuppression. Indian J Med Res 2009;130:63-6.  Back to cited text no. 4
[PUBMED]    
5.
Stark D, Barratt JL, van Hal S, Marriott D, Harkness J, Ellis JT. Clinical significance of enteric protozoa in the immunosuppressed human population. Clin Microbiol Rev 2009;22:634-50.  Back to cited text no. 5
    
6.
Rao RP. Study of opportunistic intestinal parasitic infections in HIV seropositive patients at a tertiary care teaching hospital in Karnataka, India. Int J Contemp Med Res 2016;3:2219-22.  Back to cited text no. 6
    
7.
Kashyap B, Sinha S, Das S, Rustagi N, Jhamb R. Efficiency of diagnostic methods for correlation between prevalence of enteric protozoan parasites and HIV/AIDS status – an experience of a tertiary care hospital in East Delhi. J Parasit Dis 2010;34:63-7.  Back to cited text no. 7
    
8.
Baveja UK. Manual on Laboratory Diagnosis of Common Opportunistic Infections Associated with HIV/AIDS. National Institute of Communicable Diseases (Government of India) and National AIDS Control Organization (Government of India); 2001. p. 51-2.  Back to cited text no. 8
    
9.
Government of India. Annual Report NACO. Government of India; 2016-17. p. 11-4.  Back to cited text no. 9
    
10.
Osmanov S, Pattou C, Walker N, Schwardländer B, Esparza J; WHO-UNAIDS Network for HIV Isolation and Characterization. Estimated global distribution and regional spread of HIV-1 genetic subtypes in the year 2000. J Acquir Immune Defic Syndr 2002;29:184-90.  Back to cited text no. 10
    
11.
Prasad KN, Nag VL, Dhole TN, Ayyagari A. Identification of enteric pathogens in HIV-positive patients with Diarrhoea in Northern India. J Health Popul Nutr 2000;18:23-6.  Back to cited text no. 11
    
12.
Mohandas, Sehgal R, Sud A, Malla N. Prevalence of intestinal parasitic pathogens in HIV-seropositive individuals in Northern India. Jpn J Infect Dis 2002;55:83-4.  Back to cited text no. 12
    
13.
Kumar SS, Ananthan S, Lakshmi P. Intestinal parasitic infection in HIV infected patients with Diarrhoea in Chennai. Indian J Med Microbiol 2002;20:88-91.  Back to cited text no. 13
[PUBMED]  [Full text]  
14.
Mathur MK, Verma AK, Makwana GE, Sinha M. Study of opportunistic intestinal parasitic infections in human immunodeficiency virus/acquired immunodeficiency syndrome patients. J Glob Infect Dis 2013;5:164-7.  Back to cited text no. 14
    
15.
Anand L, Dhanachand C, Brajachand N. Prevalence and epidemiologic characteristics of opportunistic and non-opportunistic intestinal parasitic infections in HIV positive patients in Manipur. J Commun Dis 1998;30:19-22.  Back to cited text no. 15
    
16.
Swathirajan CR, Vignesh R, Pradeep A, Solomon SS, Solomon S, Balakrishnan P. Occurrence of enteric parasitic infections among HIV-infected individuals and its relation to CD4 T-cell counts with a special emphasis on coccidian parasites at a tertiary care centre in South India. Indian J Med Microbiol 2017;35:37-40.  Back to cited text no. 16
[PUBMED]  [Full text]  


    Figures

  [Figure 1], [Figure 2], [Figure 3]
 
 
    Tables

  [Table 1], [Table 2]



 

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