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Figure 2: Polymerase chain reaction-based assay for typing herpes simplex virus. Extraction of viral DNA: using QIAmp® MiniElute® Virus Kit (QIAGEN). Primers: for Pol gene (IDT, India). Herpes simplex virus. Forward primer: 5'- CAG AAC TTC TAC AAC CCC CA -3'. Reverse primer: 5'- TAG ATG ATG CGC ATG GAG TA -3'

Figure 2: Polymerase chain reaction-based assay for typing herpes simplex virus. Extraction of viral DNA: using QIAmp<sup>®</sup> MiniElute<sup>®</sup> Virus Kit (QIAGEN). Primers: for <i>Pol gene</i> (IDT, India). Herpes simplex virus. Forward primer: 5'- CAG AAC TTC TAC AAC CCC CA -3'. Reverse primer: 5'- TAG ATG ATG CGC ATG GAG TA -3'